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Biological System

Immune & Inflammatory Response

How the body's defense system shifts during spaceflight

Overview

The immune system is the body's defense network — a complex web of cells and proteins that identify and neutralize threats. During spaceflight, this system undergoes measurable shifts. The Inspiration4 crew showed changes in circulating cytokine levels, white blood cell composition, and T/B-cell receptor repertoire diversity, all measured at multiple timepoints before, during (proxied by R+1), and after the 3-day mission.

Data sources: OSD-570 VDJ repertoire (TCR/BCR), PBMC snRNA-seq cell type ratios, OSD-575 71-plex cytokine panel (Alamar + Eve panels)

Key findings

  • Cytokine levels (TNFα, IL-6, IL-1β) showed individual-specific trajectories — no uniform crew-wide pattern.
    crew who showed this effect
  • TCR clonal diversity, measured via VDJ repertoire sequencing, shifted acutely at R+1 and began recovering by R+45.
    crew who showed this effect
  • WBC and neutrophil counts tracked similarly to known short-duration spaceflight patterns.
    crew who showed this effect
  • All observations reflect direction of change from each individual's own pre-mission level — not a population reference.
    crew who showed this effect
n=4 crew members · Individual differences dominate the signal

How to read this page: These findings describe four individuals at one point in time. No finding should be generalized to spaceflight health broadly. Individual differences dominate the signal. Values labeled “2× individual baseline” use derived thresholds, not clinical cutoffs.

Individual trajectories

change from own pre-mission level · n=4fold-change from L-44
C001
C002
C003
C004

All crew · Immune & Inflammatory ResponseTrajectory chart renders after preprocessing

Lines show each crew member's change from their own pre-mission level. Shaded band = typical reference range from OSD-575 data files.

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